A detailed protocol for expression, purification, and activity determination of recombinant SaCas9 - ScienceDirect
Direct purification of CRISPR/Cas ribonucleoprotein from E. coli in a single step
Cas9 Nuclease
STANDARD OPERATING PROCEDURE DETAILS
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease - ScienceDirect
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
CRISPR-Cas9 Protein – TriAltus Bioscience
Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9
CRISPR-Cas9-induced gene knockout in zebrafish
CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion | The EMBO Journal
Purification of Cas9 recombinant protein. (A) Purification of Cas9 by... | Download Scientific Diagram
The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments | Scientific Reports
Addgene: CRISPR Plasmids - Purify Genomic Loci
CRISPR-Cas9/Cas12A expression, purification and validation - Genome Innovation Hub - University of Queensland
A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
Establishment of an efficient genotyping technique based on targeted DNA endonuclease <i>in vitro</i> activity of CRISPR/Cas9 ribonucleoprotein
Production of Purified CasRNPs for Efficacious Genome Editing - Lingeman - 2017 - Current Protocols in Molecular Biology - Wiley Online Library
MPs | Free Full-Text | A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins | Communications Biology
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Isolating Genomic Regions of Interest with the CRISPR System
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins | Scientific Reports
Physicochemical and Functional Characterization of Differential CRISPR-Cas9 Ribonucleoprotein Complexes | Analytical Chemistry
Purifying Cas Proteins and Complexes: Part I- TriAltus Bioscience Blog
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9
